منابع مشابه
Hot Spring Metagenomics
Hot springs have been investigated since the XIX century, but isolation and examination of their thermophilic microbial inhabitants did not start until the 1950s. Many thermophilic microorganisms and their viruses have since been discovered, although the real complexity of thermal communities was envisaged when research based on PCR amplification of the 16S rRNA genes arose. Thereafter, the pos...
متن کاملDiversity of dsDNA Viruses in a South African Hot Spring Assessed by Metagenomics and Microscopy
The current view of virus diversity in terrestrial hot springs is limited to a few sampling sites. To expand our current understanding of hot spring viral community diversity, this study aimed to investigate the first African hot spring (Brandvlei hot spring; 60 °C, pH 5.7) by means of electron microscopy and sequencing of the virus fraction. Microscopy analysis revealed a mixture of regular- a...
متن کاملMetagenomics of an Alkaline Hot Spring in Galicia (Spain): Microbial Diversity Analysis and Screening for Novel Lipolytic Enzymes
A fosmid library was constructed with the metagenomic DNA from the water of the Lobios hot spring (76°C, pH = 8.2) located in Ourense (Spain). Metagenomic sequencing of the fosmid library allowed the assembly of 9722 contigs ranging in size from 500 to 56,677 bp and spanning ~18 Mbp. 23,207 ORFs (Open Reading Frames) were predicted from the assembly. Biodiversity was explored by taxonomic class...
متن کاملHot nuclei at the Cold Spring
Biologists are used to the idea that structure and function are interconnected; they can relate the gross anatomy of a limb to locomotion, the organization of a mitochondrion to energy production, and the geometry of an enzyme to a catalytic activity. Therefore, they would expect textbooks and conferences on the nucleus to relate the vital functions like replication and transcription to the und...
متن کاملVirus silicification under simulated hot spring conditions.
Silicification of organisms in silica-depositing environments can impact both their ecology and their presence in the fossil record. Although microbes have been silicified under laboratory and environmental conditions, viruses have not. Bacteriophage T4 was successfully silicified under laboratory conditions that closely simulated those found in silica-depositing hot springs. Virus morphology w...
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ژورنال
عنوان ژورنال: Life
سال: 2013
ISSN: 2075-1729
DOI: 10.3390/life3020308